Review



perm buffer  (fluidigm)


Bioz Verified Symbol fluidigm is a verified supplier
Bioz Manufacturer Symbol fluidigm manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    fluidigm perm buffer
    Perm Buffer, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perm+buffer/Maxpar+Perm-S+Buffer/pmc13043783-83-26-28
    Average 93 stars, based on 68 article reviews
    perm buffer - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Tumour-intrinsic features shape T cell differentiation through precursor to symptomatic multiple myeloma.
    Article Snippet: .. Cells were then AR TI CL E IN P RE SS incubated with Cell-ID intercalator-Ir (Fluidigm Sciences) to stain all cells in MaxPar Fix and Perm Buffer (Fluidigm Sciences, 201067) and aliquoted and frozen in cryovials. ..

    Article Title: The use of mass cytometry (CyTOF) to evaluate the cellular uptake of stable radiopharmaceutical surrogates in single cells: a proof-of-concept study
    Article Snippet: .. For DNA staining, required for event identification and singlet gating, cells were incubated with Cell-IDTM Intercalator-Ir (125 nM, Standard BioTools), prepared in Maxpar ® Fix and Perm Buffer (Standard BioTools). ..

    Article Title: Tumour-intrinsic features shape T cell differentiation through precursor to symptomatic multiple myeloma
    Article Snippet: .. Cells were then incubated with Cell-ID intercalator-Ir (Fluidigm Sciences) to stain all cells in MaxPar Fix and Perm Buffer (Fluidigm Sciences, 201067) and aliquoted and frozen in cryovials. ..

    Article Title: Single-cell analysis of signalling and transcriptional responses to type I interferons.
    Article Snippet: Cells were resuspended in 1.6% (v/v) formaldehyde (Pierce, #28906) diluted in Maxpar PBS and incubated for 10min at room temperature. .. Cells were resuspended in 125mM cell-ID Intercalator (Fluidigm) diluted in Maxpar Fix and Perm Buffer (Fluidigm) and incubated overnight at 4 °C. .. Compensation beads (OneComp eBeads Compensation Beads, Invitrogen, #01-111-42) stained with 1 μl of each antibody were also prepared.

    Article Title: Evaluation of nanobodies engineered with extra-cysteines for site-specific functionalization.
    Article Snippet: After washing, a freshly prepared 1.6% formaldehyde solution (Pierce, 16% Formaldehyde, 28906, Thermo Scientific) was added, and cells were incubated for 15 min at room temperature before centrifugation at 800 x g for 5 min. A barcoding strategy was applied using Cell-ID 20-Plex Pd Barcoding Kit (201060, Standard Biotools), allowing simultaneous analysis of up to 20 samples per experiment. .. Each barcode was resuspended in MaxPar Fix & Perm Buffer (201067, Standard Biotools) and transferred to the corresponding sample, followed by incubation for 30 min at room temperature and three washes with CSB at 800 x g for 5 min. Barcoded cells were then pooled together and incubated with Cell-ID Intercalator-Ir (201192 A, Standard Biotools) diluted in MaxPar Fix & Perm Buffer at a final concentration of 50 nM and stored at 4◦C overnight. .. Prior to the acquisition, cells were washed twice with CSB and twice with MaxPar Cell Acquisition Solution Plus (CAS PLUS, 201244, Standard Biotools) at 800 x g for 5 min at 4◦C, then resuspended at 6 × 105 per mL in CAS PLUS.

    Article Title: Latent Neoehrlichia mikurensis Infections May Be Reactivated in Patients With B-Cell Lymphomas Treated With Rituximab.
    Article Snippet: The samples were washed, fixed with 1.6% formaldehyde and permeabilised using Foxp3/ Transcription Factor Staining buffer (eBioscience, San Diego, CA, USA) for 60 min at RT prior to a 60 min- incubation at RT with antibodies directed against five intracellular markers (Table S3). .. The cells were then washed, incubated for 45 min at RT with 62.5 nM Cell- ID Intercalator- Ir diluted in Maxpar Fix and Perm Buffer (Fluidigm) and stored at −80°C until analysis 13652567, 0, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/im m .70120 by U niversity O f B ristol L ibrary, W iley O nline L ibrary on [09/02/2026]. ..

    Staining:

    Article Title: Tumour-intrinsic features shape T cell differentiation through precursor to symptomatic multiple myeloma.
    Article Snippet: .. Cells were then AR TI CL E IN P RE SS incubated with Cell-ID intercalator-Ir (Fluidigm Sciences) to stain all cells in MaxPar Fix and Perm Buffer (Fluidigm Sciences, 201067) and aliquoted and frozen in cryovials. ..

    Article Title: The use of mass cytometry (CyTOF) to evaluate the cellular uptake of stable radiopharmaceutical surrogates in single cells: a proof-of-concept study
    Article Snippet: .. For DNA staining, required for event identification and singlet gating, cells were incubated with Cell-IDTM Intercalator-Ir (125 nM, Standard BioTools), prepared in Maxpar ® Fix and Perm Buffer (Standard BioTools). ..

    Article Title: MIMIC: a flexible pipeline to register and summarize IMC-MSI experiments.
    Article Snippet: .. After staining, cells were washed twice with phosphate-buffered saline (PBS) and then simultaneously fixed while staining DNA using Cell-ID Intercalator Ir (Standard BioTools PN: 201192B) diluted 1:1000 in Maxpar Fix and Perm Buffer (Standard BioTools PN: 201067) for thirty minutes. ..

    Article Title: Tumour-intrinsic features shape T cell differentiation through precursor to symptomatic multiple myeloma
    Article Snippet: .. Cells were then incubated with Cell-ID intercalator-Ir (Fluidigm Sciences) to stain all cells in MaxPar Fix and Perm Buffer (Fluidigm Sciences, 201067) and aliquoted and frozen in cryovials. ..

    Saline:

    Article Title: MIMIC: a flexible pipeline to register and summarize IMC-MSI experiments.
    Article Snippet: .. After staining, cells were washed twice with phosphate-buffered saline (PBS) and then simultaneously fixed while staining DNA using Cell-ID Intercalator Ir (Standard BioTools PN: 201192B) diluted 1:1000 in Maxpar Fix and Perm Buffer (Standard BioTools PN: 201067) for thirty minutes. ..

    Concentration Assay:

    Article Title: Evaluation of nanobodies engineered with extra-cysteines for site-specific functionalization.
    Article Snippet: After washing, a freshly prepared 1.6% formaldehyde solution (Pierce, 16% Formaldehyde, 28906, Thermo Scientific) was added, and cells were incubated for 15 min at room temperature before centrifugation at 800 x g for 5 min. A barcoding strategy was applied using Cell-ID 20-Plex Pd Barcoding Kit (201060, Standard Biotools), allowing simultaneous analysis of up to 20 samples per experiment. .. Each barcode was resuspended in MaxPar Fix & Perm Buffer (201067, Standard Biotools) and transferred to the corresponding sample, followed by incubation for 30 min at room temperature and three washes with CSB at 800 x g for 5 min. Barcoded cells were then pooled together and incubated with Cell-ID Intercalator-Ir (201192 A, Standard Biotools) diluted in MaxPar Fix & Perm Buffer at a final concentration of 50 nM and stored at 4◦C overnight. .. Prior to the acquisition, cells were washed twice with CSB and twice with MaxPar Cell Acquisition Solution Plus (CAS PLUS, 201244, Standard Biotools) at 800 x g for 5 min at 4◦C, then resuspended at 6 × 105 per mL in CAS PLUS.



    Similar Products

    86
    10X Genomics perm buffer
    Perm Buffer, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perm+buffer/buffer+perm/pm42106323-210-2-4
    Average 86 stars, based on 1 article reviews
    perm buffer - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    93
    fluidigm perm buffer
    Perm Buffer, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perm+buffer/Maxpar+Perm-S+Buffer/pmc13043783-83-26-28
    Average 93 stars, based on 1 article reviews
    perm buffer - by Bioz Stars, 2026-09
    93/100 stars
      Buy from Supplier

    95
    fluidigm maxpar barcode perm buffer
    Maxpar Barcode Perm Buffer, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perm+buffer/Maxpar+Barcode+Perm+Buffer/bio_rxiv__64898__2026__03__23__713704-220-70-74
    Average 95 stars, based on 1 article reviews
    maxpar barcode perm buffer - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    fluidigm perm solution
    Perm Solution, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perm+buffer/Maxpar+Fix+and+Perm+Buffer/bio_rxiv__64898__2026__03__21__713366-288-24-26
    Average 95 stars, based on 1 article reviews
    perm solution - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    97
    Cytek Biosciences foxp3 fix perm buffer
    Foxp3 Fix Perm Buffer, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perm+buffer/Foxp3+%2F+Transcription+Factor+Staining+Buffer+Kit/pm41839922-98-12-14
    Average 97 stars, based on 1 article reviews
    foxp3 fix perm buffer - by Bioz Stars, 2026-09
    97/100 stars
      Buy from Supplier

    95
    Cytek Biosciences 1x permwash
    1x Permwash, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perm+buffer/Flow+Cytometry+Perm+Buffer/pm41839922-98-27-29
    Average 95 stars, based on 1 article reviews
    1x permwash - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    fluidigm cell staining buffer 784
    Cell Staining Buffer 784, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/perm+buffer/Maxpar+Barcode+Perm+Buffer/pm41834128-345-4-12
    Average 95 stars, based on 1 article reviews
    cell staining buffer 784 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    Image Search Results